P2-163 Natural Occurrence of HT-2 and T-2 Toxin and Its Production of F. armeniacum Isolated in Rice

Tuesday, August 2, 2016
America's Center - St. Louis
Jae-Gee Ryu, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Korea, The Republic of
Sung Kee Hong, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Korea, The Republic of
Theresa Lee, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Korea, The Republic of
Soohyung Lee, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Korea, The Republic of
Hyeonheui Ham, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Korea, The Republic of
Hye Yeon Mun, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Korea, The Republic of
Introduction: T-2 toxin and HT-2 toxin are mycotoxins of the group trichothecenes type A produced by the Fusarium genus which are commonly found in various cereal crops. T-2-and HT-2 toxin often occur together in infected cereals and their contamination of human foods and animal feeds is a continuing worldwide problem. 

Purpose: The purpose of this study was to illustrate the mycotoxin production of causal fungus associated with natural occurrence of T-2 and HT-2 toxin in rice.

Methods: Identification and incidence of T-2 toxin producing Fusarium armeniacum isolates and natural occurrence of T-2 toxin and HT-2 toxin in 2012–2013 rice grains collected in Korea. Mycotoxins produced by the Fusarium isolates and rice grain samples were detected with LC-MS.

Results: HT-2 toxin only was found in polished rice sample with 0.02 ppb (n=1) in 2012 and unpolished 0.1 ppb (n=4), brown 0.1-0.2 ppb (n=2), polished 0.1-0.2 ppb (n=3) in 2013. F. armeniacum isolated from grain samples contaminated with HT-2 toxin. In total 526 Fusarium isolates, F. armeniacum was 50 isolates. To investigate mycotoxin production of these isolates (n=15) were cultured in rice grain for 21days. T-2 and HT-2 toxin of these isolates were detected with 173.6-414.9 ppm, 42.0–76.0 ppm.

Significance: Our study suggest that trichothecene A type in rice will be problem depend on environmental condition in Korea. The further study on monitoring Fusarium contamination and natural occurrence of mycotoxin should be continued.