P2-04 Lactobacillus rhamnosus GG Inhibits BID-dependent Apoptosis in Human Hepatocellular Carcinoma Cells Exposed to Patulin

Tuesday, July 11, 2017
Exhibit Hall (Tampa Convention Center)
Bernice Karlton-Senaye , North Carolina A&T State University Center of Postharvest Technologies (CEPHT) , Kannapolis , NC
Rishipal Bansode , North Carolina A&T State University Center of Postharvest Technologies (CEPHT) , Kannapolis , NC
Priscilla Randolph , North Carolina A&T State University Center of Postharvest Technologies (CEPHT) , Kannapolis , NC
Leonard Williams , North Carolina A&T State University-Center of Postharvest Technologies (CEPHT) , Kannapolis , NC
Introduction: Patulin, a mycotoxin, which is a major contaminant in apple juices, has contributed immensely to the occurrence of liver diseases. Consumption of apple juice could, over long period of time, become harmful to the health of individuals with pre-existing liver disease. Probiotics are known for their role in patulin removal from aqueous media.

Purpose: The purpose of this study was to determine the effects of a probiotic microorganism on patulin toxicity in hepatocellular carcinoma (HepG2) cells and established the protective effect of Lactobacillus rhamnosusas mediated by induction of BID in response to patulin toxicity.

Methods: HepG2 cells were seeded in 24 well plates (105 cells/well) in EMEM containing 10% fetal bovine serum for 24 h to ensure cell adherence. Cells were exposed to patulin at 0, 1, 2.5, 5, 7.5, and 10 µM for 24 h followed by treatment with Lactobacillus rhamnosus GG (LGG) for 24 h. Total protein normalization and western blot were conducted to determine the expression of PUMA and BID.

Results:  After 24 h of patulin exposure, followed by 24 h of treatment with Lactobacillus rhamnosus (LGG), cells proliferation decreased with increasing patulin exposure in samples without LGG pretreatment; whereas, with increasing concentration of patulin, cells were relatively rescued in LGG treated samples. It was further observed that pretreatment of LGG with polysaccharide gums led to a decline in cell proliferation with increasing patulin exposure. Compare to the control, the expression of PUMA increased slightly, by 7%, at 10µM patulin exposure in treatment. However, the expression of BID decreased by 26% in treatment compared to the control showing the protective effect of LGG.

Significance: Our findings suggest that LGG could potentially function as a therapeutic agent to reverse the damaging effect of patulin on the liver of individuals with pre-existing liver disease.