Purpose: This study examined the influence of persistent and transient generic E. coli genotypes and Salmonella spp., recovered from a beef packing plant, on biofilm formation of E. coli O157:H7.
Methods: Biofilm formation of five genotypes of persistent and transient generic E. coli and a strain of Salmonella spp. was tested alone or in dual-cultures with E. coli O157:H7 at 15°C for up to six days. Biofilms were quantified using Crystal Violet (CV) staining on day 2, 4, and 6. The numbers of E. coli O157:H7 in mono- or dual-culture biofilms with persistent and transient E. coli genotypes 390 and 533, respectively, and with Salmonella spp. were determined by plating on selective agar.
Results: Strain by day interaction was found (P<0.0001) when biofilm formation of E. coli genotypes and Salmonella spp. were cultured alone or with E. coli O157:H7. In monoculture biofilm, the numbers of E. coli O157:H7 ranged between 5.87 and 6.69 log CFU at ≤ six days. Escherichia coli genotype 136 significantly (P<0.05) reduced the numbers of E. coli O157 in dual-culture biofilms by ≥1.5 log CFU at ≥ two days, while genotype 533 caused ≥ one log CFU reduction at ≥ four days. Salmonella spp. did not affect (P>0.05) the numbers of E. coli O157:H7 in dual-culture biofilm.
Significance: The finding that generic E. coli genotypes significantly reduced biofilm formation by E. coli O157:H7 suggested that generic E. coli may potentially be used as an agent to control E. coli O157:H7 in beef processing environment.