Purpose: The objective of this study was to determine the effects of temperature and packaging materials on the growth/survival of Salmonella on waxberry during storage.
Methods: Waxberry samples inoculated with Salmonella spp. cocktails (ATCC 14028, 50335) were storied at 4°C, 16°C, and 33°C for 72 h, using two package materials of plastic sealing bags and preservative film, respectively. Bacterial populations were enumerated every eight hours. Primary models (log-linear, Gompertz, and Weibull) were used to fit curves, and statistic criteria of goodness-of-fit such as mean square error, RMSE were used for model evaluation.
Results: A reduction of two log CFU/g of Salmonella spp. on waxberry was observed after 72 h storage at 4°C, while a more rapid decrease was found with the preservative film packaging with plastic sealing bags (P<0.01). The lag phase of Salmonella spp. on waxberry was up to 40 h at storage temperatures of 16°C and 33°C and then an increase of one to two log CFU Salmonella spp./g was observed. The prevention of bacterial growth with the packages with preservative film was greater than that with plastic sealing basg (P<0.01). The log-linear model performed better for describing the bacterial growth/survival curves at three different temperatures, as demonstrated by the statistical indices (R2>0.9, P<0.05, RMSE<0.15).
Significance: This study provided a predictive model for evaluation of the storage methods for waxberry.