Purpose: The purpose of this study was to evaluate the synergistic effect of curcumin and AA in inactivating Gram positive and Gram negative foodborne pathogens.
Methods: Curcumin was dissolved in 95% EtOH and diluted to desired concentrations with sterile distill water (SDW). AA was dissolved in SDW and filter sterilized. Overnight cultures of L. monocytogenes L008 or E. coli O157:H7 86-24 were diluted to 6.0 log CFU/ml. Curcumin, AA, and cells were mixed in amber tubes and pre-incubated in the dark for 5 min. The mixture was then transferred to a 96-well plate and illuminated (450-455 nm, 0.13 W/cm2) for 1 min (L. monocytogenes) or 10 min (E. coli O157:H7). Number of viable cells was determined; the detection limit was 2.4 log CFU/ml. Results were compared by ANOVA.
Results: A statistically significant, synergistic effect was observed for curcumin/AA concentrations (p < 0.05) in the inactivation of each pathogen. Based on direct plating, no viable L. monocytogenes were detected when exposed to 10 uM of curcumin and 250 ug/ml of AA; while E. coli O157:H7 were below detection limit following treated with 50 uM of curcumin and 25 ug/ml of AA. Populations of E. coli O157:H7 were 4.3 log CFU/ml and 5.9 log CFU/ml when exposed to 50 uM curcumin or 25 ug/ml AA, respectively.
Significance: The combination of photosensitizing curcumin and ascorbic acid has potential application as an antimicrobial treatment for inactivation of foodborne pathogens on the surface of meat, poultry, and fresh produce.