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P2-159 Comparison of Two Diagnostic Methods to Detect Five Different Bacterial Pathogens Associated with Porcine Respiratory Disease Complex (PRDC) and Investigation of Their Prevalence in Pathologic Lung Tissues in Korea

Tuesday, July 11, 2017
Exhibit Hall (Tampa Convention Center)
Kun Taek Park , Seoul National University , Seoul , South Korea
Chan Hee Lee , Seoul National University , Seoul , South Korea
Young Kyung Park , Seoul National University , Seoul , South Korea
Chung Wung Kim , Seoul National University , Seoul , South Korea
Sook Shin , Seoul National University , Seoul , South Korea
Yong Ho Park , Seoul National University , Seoul , South Korea
Introduction: Porcine respiratory disease complex (PRDC) greatly affects the health and production of pigs. PRDC is polymicrobial disease caused by infection of various combinations of primary and secondary bacterial pathogens including Pasteurella multocida (PM), Mycoplasma hyopneumoniae (MH), Streptococcus suis (SS), Actinobacillus pleuropneumoniae (APP), and Haemophilus parasuis (HP). It is important to investigate the relative prevalence of bacterial pathogens in PRDC and efficient identification methods to control the disease.

Purpose: The current study was performed to compare two diagnostic methods to detect the five causative bacterial pathogens in lung tissues and to investigate the distribution of the bacterial pathogens in Korea.

Methods: A total of 271 lung samples with pathologic lesion were obtained from two slaughterhouses in Korea. Two diagnostic methods were used to detect the bacterial pathogens: i) PCR following DNA extraction from tissues, and ii) Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) following bacterial culture on blood agar.

Results: Among 271 tissue samples, PCR detected all the five pathogens, with the highest detection rate for MH (n = 141, 52%) followed by SS (14, 5.1%), HP (5, 1.8%), APP (5, 1.8%), and PM (4, 1.4%). In contrast, MALDI-TOF following culture method detected only SS (29, 10.7%), PM (29, 10.7%), and APP (1, 0.3%). When the results are combined, the overall detection rates were, from highest to lowest: MH (141, 52%) followed by PM (33, 12.2%), SS (29, 10.7%), APP (6, 2.2%), and HP (5, 1.8%).

Significance: The results indicate that the use of a combinational method of PCR and culture might be a useful way to increase the sensitivity for detection of PRDC pathogens and that the predominant bacterial pathogens for PRDC in Korea are MH and PM. This study provides useful information to control the diseases, which will increase the productivity and animal health on pig farms.

Back to: Poster Session 2 – Food Chemical Hazards and Food Allergens, Food Toxicology, Meat, Poultry and Eggs, Epidemiology, Retail and Food Service Safety, Communication Outreach and Education, Molecular Analytics, Genomics and Microbiome, Laboratory and Detection Methods, and Dairy
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