Purpose: The objective of this study was to determine the D- and z-values of Staphylococcus aureus in whey.
Methods: Six lots of whey (three lots each from cheese made with or without mesophilic starter culture) were standardized to pH 6.5+0.1 with NaOH and inoculated with a three-strain mixture of S. aureus to yield nine log CFU/ml. Samples (1ml aliquots) were vacuum-sealed in moisture-impermeable pouches and heated to 60.0, 62.8, 65.6, or 68.3°C (140, 145, 150, or 155°F) by submersion in a water bath. For each treatment, duplicate samples were removed from heating at appropriate time intervals and enumerated for surviving S. aureusby plating on Baird-Parker agar overlaid with Tryptic Soy agar to aid in the recovery of heat-injured cells.
Results: There was no statistical differences in thermal inactivation rates for whey, regardless of whether it was produced with starter culture (P>0.05). Pooled data was used to calculate thermal inactivation rates. D-values were 1.32, 0.38, 0.12, and 0.07 minutes for samples heated at 60.0, 62.8, 65.6, or 68.3°C, respectively. The z-value was calculated to be 6.1°C (11.56°F) with a best-fit reference temperature of 62.8°C.
Significance: These data can be used by the dairy industry to develop pasteurization procedures to kill vegetative pathogens such as S. aureus.