Purpose: Evaluation of microbial composition changes in chicken cecal contents after vaccine administration to assess potential impacts of the Salmonella Typhimurium vaccine strains.
Methods: The cecal microbial communities of unvaccinated (group A) and vaccinated chickens (group 2, vaccinated with PBAD-mviN; group 3, vaccinated with wild type; group 4, vaccinated with DDmetRmetD) were compared through denaturing gradient gel electrophoresis (DGGE) and microbiome sequencing analysis.
Results: Chickens from different treatment groups exhibited DGGE patterns that were indistinguishable from each other. However, microbiome sequencing analysis of individual chicken ceca revealed considerable changes in microbial composition by different vaccine strain treatments and detectable patterns of distinctive clustering among groups. The PBAD-mviN Salmonella Typhimurium yielding the highest vaccine efficacy led to the most distinctive changes in the cecal microbiota.
Significance: The present study provides comprehensive information about the impact of vaccine strains which can effectively control Salmonella Typhimurium in poultry on the microbiota of host and it may contribute to better understanding of vaccine strain effects.