Purpose: To perform a comparative evaluation of ECO methods against the USDA/FSIS MLG 5.09 and 5A.04.
Methods: In total, 451 mTSB enriched beef and beef products samples were processed through MLG and ECO. All positive results were confirmed using culture-based isolation of STEC O157. Any discrepant results were retested for confirmation.
Results: Comparative sensitivity, specificity, false positive rate, and false negative rate against culture between MLG and ECO were 91 vs 92, 91 vs 99%, 9 vs 0.76%, and 8.5 vs 8.3%, respectively. Positive predictive value, negative predictive value and the overall accuracy were found to be 54 vs 95, 99 vs 99%, and 91 vs 99%, for MLG and ECO, respectively.
Significance: These data demonstrate that the ECO assay provides a highly sensitive detection of Escherichia coli O157:H7 that is comparable to USDA/FSIS MLG. The convenient sample preparation along with a user friendly instrumentation and software interface, makes the GENE-UP™ E. coli O157:H7 a viable alternative for Escherichia coli O157:H7 detection.