Purpose: Thus the purpose of this study was to evaluate the efficiency of two disinfectants Vital Oxide and hydrogen peroxide delivered as fog and vapor form against the Klebsiella pneumoniae, Escherichia coli, and S. aureus on stainless steel contact surface exposes for 10, 15, and 20 minutes.
Methods: The bacterium were dried on stainless steel coupons and placed in three different locations in lab. Two different room decontamination machines were use to deliver the disinfectants for 10, 15, and 20 minutes. After the exposure, stainless steel coupons were collected and immersed in three ml of saline, 20 μl of saline were sampled with bacteria and plated, allow to dry, incubated over night and colonies were counted. Efficacy of disinfectant were calculated and compared to controls by Tukey’s studentized range test.
Results: Outcome of this experiment suggest that efficacy of disinfectants depends on the time, even though Vital Oxide was significantly (P<0.05) more effective (1.15±0.03) in reducing K. pneumonia than S. aureus with 10 minute of exposure compared to the other treatments and controls.
Significance: These data suggest that Vital Oxide showed more antibacterial activities against the CRE than MRSA, which can be used as a alternative chemicals to decontamination of equipment and health care facilities.