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P3-212 A Method for the Improved Detection of Aerosolized Influenza Viruses Using Impingers That Incorporate Anion Exchange Resin

Wednesday, July 12, 2017
Exhibit Hall (Tampa Convention Center)
Jeffrey Chandler , U.S. Department of Agriculture-APHIS-WS-NWRC , Fort Collins , CO
Joshua Schaeffer , Colorado State University , Fort Collins , CO
Margaret Davidson , Colorado State University , Fort Collins , CO
Sheryl Magzamen , Colorado State University , Fort Collins , CO
Alma Perez-Mendez , Leprino Foods Company , Denver , CO
Stephen Reynolds , Colorado State University , Fort Collins , CO
Lawrence Goodridge , McGill University , Montreal , Canada
John Volckens , Colorado State University , Fort Collins , CO
Alan Franklin , U.S. Department of Agriculture-APHIS-WS-NWRC , Fort Collins , CO
Susan Shriner , U.S. Department of Agriculture-APHIS-WS-NWRC , Fort Collins , CO
Bledar Bisha , University of Wyoming , Laramie , WY
Introduction: The important role of bioaerosols in influenza transmission is well established, as highlighted by the 2014-2015 outbreak of highly pathogenic avian influenza in U.S. poultry production. Accordingly, active sampling of bioaerosols for influenza (and other microorganisms) is suggested as an important mitigation tool.

Purpose: Here, we adapted an anion exchange resin-based method, initially developed to improve the detection of negatively-charged viruses in water, to impingement-based bioaerosol sampling of influenza viruses.

Methods: Type A and type B influenza viruses contained within the FluMist Quadrivalent vaccine were aerosolized to the standardized particle density of 5 mg/m3 using a 6-jet collision nebulizer within a customized bioaerosol chamber.  Aerosols were generated using 10-fold serial dilutions of viral inocula ranging in concentration from 10-2.5 to 103.5 FFU/ml.  For each experimental condition (tested in triplicate), two SKC BioSamplers containing 20 ml of PBS with and without 0.5 g of IRA-900 anion exchange resin were co-located within the chamber and calibrated to sample 500 liters of the bioaerosol.  RNA was then isolated from the PBS used for impingement and from IRA-900 in preparation for real time reverse transcriptase PCR (RT-PCR) analyses.

Results: The anion exchange resin-based method improved detection of type A and type B influenza viruses by an average of 6.77× and 3.33× (p<0.05), respectively. Both type A and type B influenza viruses were detected more effectively at higher inoculum concentrations. Detection was improved by 9.55× for type A and 6.98× for type B influenza viruses when the viral inoculum used for nebulization was at a concentration of 103.5FFU/ml.

Significance: A bioaerosol sampling method for influenza viruses was developed that allowed for improved molecular detection, was simple to perform, and adaptable to existing bioaerosol sampling equipment.

Back to: Poster Session 3 – Low-water Activity Foods, Packaging, Produce, Microbial Food Spoilage, Antimicrobials, Laboratory and Detection Methods, Water
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