Purpose: Evaluate the ability of various STEC serotypes to survive in dry flour.
Methods: Representative serotypes from four STEC serogroups (O45, O103, O145, and O157) were used to inoculate separate samples of sterilized all-purpose flour. Flour was stored in an environmental chamber (25°C, RH=57%) to simulate a dry storage environment, then sampled routinely for water activity measurement and enumeration of STEC. All experiments were independently replicated (n=9).
Results: After five weeks of storage, O45 and O145 serotypes showed the greatest survival in flour with log reductions of 0.63 (±0.47) log CFU/g and 0.77 (±0.48) log CFU/g, respectively. Populations of O157 and O103 had significantly reduced (P<0.05) over five weeks with 1.2 (±0.29) log CFU/g and 1.2 (±0.26) log CFU/g, respectively. After six weeks, all serotypes except O45 were below the limit of detection (50 CFU/g), but positive after enrichment. After seven weeks, all serotypes were only positive by enrichment.
Significance: These data suggest that STEC can remain viable over many weeks stored at room temperature in a low aw environment. More studies will be needed to further characterize the survival of other STEC serotypes in this environment for longer periods of time.