Wednesday, May 11, 2016
Megaron Athens International Conference Center
Si Myung Lee, Division of Biosafety, National Institute of Agricultural Science, Rural Development Administration, Jeounju, Korea, The Republic of
Won Hee Lee, Division of Biosafety, National Institute of Agricultural Science, Rural Development Administration, Jeounju, Korea, The Republic of
Seon Woo Oh, Division of Biosafety, National Institute of Agricultural Science, Rural Development Administration, Jeounju, Korea, The Republic of
Soo Youn Park, Division of Biosafety, National Institute of Agricultural Science, Rural Development Administration, Jeounju, Korea, The Republic of
Hyun Suk Cho, Division of Biosafety, National Institute of Agricultural Science, Rural Development Administration, Jeounju, Korea, The Republic of
Introduction: Rice is the staple food of Asian countries including Korea. Though rice is known to be a relatively low allergenic food and there are few reports of rice allergies, an increase in the number of patients sensitized to rice allergen with or without clinical symptoms has been reported recently. There are some reports about the protein allergens as the major IgE-binding components in rice. However, the rice allergen contents and the immunologic characteristics of rice allergen are unclear. The aim of this study was to identify the rice allergen and differences between Korean cultivars.
Purpose: This study was designed to determine the contents of major allergens in Korean rice cultivars. Further, we would like to analyze correlation between allergen contents and significance.
Methods: Among the known allergens, we selected two major allergens of rice, glyoxalase 1 and ¨»-amylase inhibitor. After the production of major allergens by genetic expression, antibodies were made for each allergen. Twenty Korean cultivars were cultivated and collected. After the extraction of total protein from the grains, we examined the contents of each allergen by ELISA and compared between cultivar. All of these data were reviewed.
Results: The selected allergen contents of Korean rice cultivars were measured. Some rice cultivars had higher allergen content than others. The Nakdong and Sobi cultivars had the largest content of glyoxalase in grain which was about 500 ng/g dry weight. The average glyoxalase contents were 322 ng/g dry weight. In a ¨»-amylase inhibitor, Kumoh and Nakdong cultivars had larger contents than others. The average ¨»-amylase inhibitor contents were 105 ng/g dry weight.
Significance: The contents of major allergens were measured in Korean rice cultivars. These results can be used for food safety assumptions and identifying allergenic reactions.
