P1-17 Optimization of Microbiological Recovery from Surfaces for Environmental Monitoring

Wednesday, May 11, 2016
Megaron Athens International Conference Center
Guido Finazzi, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy
Marina Nadia Losio, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Brescia, Italy
Giorgio Varisco, National Reference Centre for Emerging Risk in Food Safety, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “B. Ubertini”, Brescia, Italy
Introduction: The FLOQSwab is a specimen collection device recognized worldwide for its superior performance in the clinical diagnostics. The aim of this work was to evaluate the FLOQSwab for the recovery of microbiological samples from surfaces compared to the traditional swab (rayon tipped swab) as per ISO 18593:2004 standard.

Purpose: The FLOQSwab, thanks to the innovative manufacturing technology, allows improvements to the efficiency of recovery and release of analyte. The study has been divided into 2 experiments.

Methods: In the first experiment the two swabs were evaluated for their capacity to recover and release the analyte (three different bacterial loads of E. coli). In the second experiment, the two swabs were evaluated for their capacity to recover three different bacterial loads of E. coli from two different surface materials (stainless steel and polypropylene).

Results: In all experiments the flocked swab demonstrated a higher recovery rate compared to the traditional rayon tipped swab.

Significance: The data obtained from this preliminary study demonstrated that the FLOQSwab can be a good food surfaces collection device that improves the recovery of the analyte and thus produce accurate results. Based on the outcomes of the study, a larger field study is in progress using the FLOQSwab for sample collection to improve both environmental monitoring and the efficacy of the hygiene controls for food safety