Purpose: This study was conducted to independently evaluate an alternative rapid method to the FDA/BAM Chapter 4A method for spinach (200g), the USDA/FSIS MLG 5.09 method for raw beef trim (375 g), and the USDA/FSIS MLG 5B.05 method for raw beef trim (375 g) and raw ground beef (25g and 375g) as part of the AOAC RI™ PTM validation process.
Methods: Using 30 unpaired samples, 5 replicates were evaluated at a high inoculation level, 20 at a low inoculation level, and 5 at an uninoculated control level. Samples were analyzed by the alternative method, and confirmed according to the reference method and a proprietary alternative procedure.
Results: Results for the assay were analyzed by POD statistical analysis. No statistically significant differences were observed between the new method and the reference methods.
Significance: The data from the study demonstrated that the GENE-UP® EHEC was a rapid and reliable method for the for the detection of non-O157 STEC Top 6 and E. coli O157:H7.