Purpose: To independently evaluate the alternative rapid method, to the USDA-FSIS 8.09 method for deli ham (25g), to the FDA/BAM Chapter 10 method for smoked salmon (25 g), and the AOAC 993.12 method for Mexican soft cheese (125 g) as part of the AOAC RI™ PTM validation process.
Methods: Using 30 unpaired samples for each matrix, 5 replicates were inoculated at a high inoculation level, 20 at a low inoculation level and evaluated along with 5 uninoculated control replicates. After sample enrichment in LPT Broth, test portions were evaluated by both the alternative and reference methods. Samples were confirmed following procedures outlined in the USDA/FSIS-MLG 8.09, FDA/BAM Chapter 10, AOAC 993.12, and bioMérieux’s alternative confirmation procedure.
Results: Results for the assay were compared to the MLG, FDA, and AOAC reference methods by POD statistical analysis. No statistically significant differences were observed between the new method and the reference methods in the 3 foods.
Significance: The data from the study, demonstrates that the GENE-UP Listeria monocytogenes Assay is a rapid and reliable method for the detection of Listeria monocytogenes in the food matrices analyzed.