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P3-203 An Independent Evaluation of the GENE-UP Listeria species Assay for the Detection of Listeria Species in Foods and Environmental Surfaces

Wednesday, July 12, 2017
Exhibit Hall (Tampa Convention Center)
Patrick Bird , Q Laboratories, Inc. , Cincinnati , OH
Benjamin Bastin , Q Laboratories, Inc. , Cincinnati , OH
Joe Benzinger , Q Laboratories, Inc. , Cincinnati , OH
Erin Crowley , Q Laboratories, Inc. , Cincinnati , OH
James Agin , Q Laboratories, Inc. , Cincinnati , OH
David Goins , Q Laboratories, Inc. , Cincinnati , OH
Introduction: The ability of Listeria has to survive in various climates, such as low temperature and high pH, can cause severe problems for food manufacturers as the organism can survive cleaning conditions and contaminate food commodities. Recent regulations have increased the need for Listeria sampling programs in most food manufacturing industries.  There is a need for rapid and accurate assays that can quickly detect Listeria from environmental samples and food commodities.  The GENE-UP™ Listeria species Assay utilizes Real-Time PCR technology to provide next day results for Listeria detection.

Purpose: To independently evaluate the alternative rapid method to the USDA-FSIS 8.09 method for deli ham (125g) and stainless steel environmental surface (4” x 4” test area), to the FDA/BAM Chapter 10 method for smoked salmon (25 g), and the AOAC 993.12 method for Mexican soft cheese (25 g) as part of the AOAC RI™ PTM validation process.

Methods: Using 30 unpaired samples for each matrix, 5 replicates were inoculated at a high inoculation level, 20 at a low inoculation level and evaluated along with 5 uninoculated control replicates. After sample enrichment in LPT Broth, test portions were evaluated by both the alternative and reference methods.  Samples were confirmed following procedures outlined in the USDA/FSIS-MLG 8.09, FDA/BAM Chapter 10, AOAC 993.12, and bioMérieux’ s alternative confirmation procedure.

Results: Results for the assay were compared to the MLG, FDA, and AOAC reference methods by POD statistical analysis.  No statistically significant differences were observed between the new method and the reference methods in the 3 foods and 1 environmental surface. 

Significance: The data from the study, demonstrates that the GENE-UP™ Listeria species Assay is a rapid and reliable method for the detection of Listeria species in the food matrices and stainless steel environmental surface (4” x 4” test area) analyzed.

Back to: Poster Session 3 – Low-water Activity Foods, Packaging, Produce, Microbial Food Spoilage, Antimicrobials, Laboratory and Detection Methods, Water
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