Purpose: This study was undertaken to develop a probability model to estimate the death probability of a S. enterica Typhimurium population under conditions of desiccation over time.
Methods: Bacterial cells were prepared with an initial concentration adjusted to 1×10n CFU/μl (where n=1, 2, 3, 4, 5) by 10-fold dilution. The inocula was dispensed into 96-well microplates (2 µl per well). The microplates were stored under desiccating conditions (10 to 20% relative humidity) at 5, 15, or 25°C. The survival of bacterial cells in each well was assessed by adding 100 μl of tryptic soy broth as an enrichment culture at arbitrary time intervals.
Results: The changes in the death probability of 96 replicate bacterial populations were described as cumulative gamma distribution. Variability in time to inactivation was described to transform a cumulative gamma distribution into a gamma distribution. In addition, the certainty levels were described for bacterial inactivation that ensure death probability of a bacterial population at six different levels, from 90 to 99.9999%.
Significance: The study results supported the use of the model for describing death probability of a bacterial population. Death probability is useful in risk assessments that estimate bacteria remaining after inactivation processes.